Fields of Research
- Drug Discovery
- Ubiquitin Proteasome System
- Protein Structure
Research Summary
Developing therapeutics to rewire the ubiquitin proteasome system for targeted protein degradation.
Research Statement
I work on characterizing and determining the structures of a series of newly identified human ubiquitin ligases involved in recognizing substrate protein for polyubiquitination and degradation in protein quality control and signaling. I plan on developing and executing high-throughput screening assays to search for small molecule compounds that are capable of inducing the polyubiquination and degradation of several human disease-related proteins by the ubiquitin ligases I am characterizing. The overarching goal of my project is to translate the latest findings of structures and functions of human ubiquitin ligases for therapeutic development.
Awards and Honors
Presentation: Ubiquitins, Autophagy, and Disease National Meeting, 2019, Cold Spring Harbor Laboratories
Presentation: Pharmacology Departmental Retreat, 2018, Bainbridge Island
Posters: HHMI Science Meeting 2018, Protein Society 33 Annual Symposium 2019
Domnita Rusnac
Postdoctoral Fellow
Affiliations
UW Department of Pharmacology
Biological Physics Structure and Design (BPSD)
Domnita Rusnac
Postdoctoral Fellow
Affiliations
UW Department of Pharmacology
Biological Physics Structure and Design (BPSD)
Faculty
- Building:
- HSB
- Room:
- D408
- Box:
- 357280
- Phone:
- (206) 616-3887
- Web Link:
- https://depts.washington.edu/zhenglab/
Lab
- Building:
- HSB
- Room:
- D408
- Box:
- 357280
- Phone:
- (206) 616-3887
- Web Link:
- https://depts.washington.edu/zhenglab/
Domnita Rusnac
Postdoctoral Fellow
Affiliations
UW Department of Pharmacology
Biological Physics Structure and Design (BPSD)
Domnita Rusnac
Postdoctoral Fellow
Affiliations
UW Department of Pharmacology
Biological Physics Structure and Design (BPSD)
Domnita Rusnac
Postdoctoral Fellow
Affiliations
UW Department of Pharmacology
Biological Physics Structure and Design (BPSD)
Select Publications
Rusnac, D.-V., et al., (2018). Recognition of the Diglycine C-End Degron by CRL2KLHDC2 Ubiquitin Ligase. Molecular Cell 72, 813 – 822. (COVER story)
Lin, H.-C., Yeh, C.-W., Chen, Y.-F., Lee, T.-T., Hsieh, P.-Y., Rusnac, D. V., Lin, S.-Y., Elledge, S. J., Zheng, N. and Yen, H.-C. S. (2018). C-terminal end-directed protein elimination by CRL2 ubiquitin ligases. Molecular Cell 70, 602-613.
Canzani, D., Rusnac, D.-V., Zheng, N., Bush, M. F. (2019 in press) Degronomics: Mapping the Interacting Peptidome of a Ubiquitin Ligase Using an Integrative Mass Spectrometry Strategy Analytical Chemistry
Rusnac D.V., Zheng N. (2018) Overview of Protein Degradation in Plant Hormone Signaling. In: Hejátko, J., Hakoshima, T. (eds) Plant Structural Biology: Hormonal Regulations. Springer, Cham
Rusnac D.V., Zheng N. (2019 in press) Structural Biology of CRL Ubiquitin Ligases. In: Sun, Y., Wei, W., Jin, J. (eds) Cullin-RING Ligases and Protein Neddylation: Biology and Therapeutics
Publications
Domnita Rusnac
Postdoctoral Fellow
Affiliations
UW Department of Pharmacology
Biological Physics Structure and Design (BPSD)