Fields of Research
- Drug Discovery
- Ubiquitin Proteasome System
- Protein Structure
Developing therapeutics to rewire the ubiquitin proteasome system for targeted protein degradation.
I work on characterizing and determining the structures of a series of newly identified human ubiquitin ligases involved in recognizing substrate protein for polyubiquitination and degradation in protein quality control and signaling. I plan on developing and executing high-throughput screening assays to search for small molecule compounds that are capable of inducing the polyubiquination and degradation of several human disease-related proteins by the ubiquitin ligases I am characterizing. The overarching goal of my project is to translate the latest findings of structures and functions of human ubiquitin ligases for therapeutic development.
Awards and Honors
Presentation: Ubiquitins, Autophagy, and Disease National Meeting, 2019, Cold Spring Harbor Laboratories
Presentation: Pharmacology Departmental Retreat, 2018, Bainbridge Island
Posters: HHMI Science Meeting 2018, Protein Society 33 Annual Symposium 2019
Rusnac, D.-V., et al., (2018). Recognition of the Diglycine C-End Degron by CRL2KLHDC2 Ubiquitin Ligase. Molecular Cell 72, 813 – 822. (COVER story)
Lin, H.-C., Yeh, C.-W., Chen, Y.-F., Lee, T.-T., Hsieh, P.-Y., Rusnac, D. V., Lin, S.-Y., Elledge, S. J., Zheng, N. and Yen, H.-C. S. (2018). C-terminal end-directed protein elimination by CRL2 ubiquitin ligases. Molecular Cell 70, 602-613.
Canzani, D., Rusnac, D.-V., Zheng, N., Bush, M. F. (2019 in press) Degronomics: Mapping the Interacting Peptidome of a Ubiquitin Ligase Using an Integrative Mass Spectrometry Strategy Analytical Chemistry
Rusnac D.V., Zheng N. (2018) Overview of Protein Degradation in Plant Hormone Signaling. In: Hejátko, J., Hakoshima, T. (eds) Plant Structural Biology: Hormonal Regulations. Springer, Cham
Rusnac D.V., Zheng N. (2019 in press) Structural Biology of CRL Ubiquitin Ligases. In: Sun, Y., Wei, W., Jin, J. (eds) Cullin-RING Ligases and Protein Neddylation: Biology and Therapeutics