Fields of Research
Intracellular Signaling
Molecular Basis of Disease
Research Summary
Stress-induced cellular inclusions: are they liquid-liquid phase separation? Do their kinetics change in aged cells? Let’s find out!
Research Statement
My training as a research scientist has taught me to view protein folding as a dynamic process that affects every single thing that happens in the cell. As a postdoc, I’ve chosen to focus on how protein “misfolding” can be an adaptive mechanism for cells challenged with environmental stresses. Specifically, I’m building on work pioneered in the Gardner lab that interrogates the yeast stress-responsive puncta formation and SUMOylation of the transcriptional repressor Cyc8. I aim to establish this protein as a model of stress-induced adaptive liquid-liquid phase separation. I am also interested in how these phenomena change as cells age.
Awards and Honors
Timothy Mackie
Postdoctoral Fellow
Affiliations
Ph.D. in Molecular, Cell, and Developmental Biology, University of Pittsburgh (2012-2018)
B.S. in Biology, Messiah College (2006-2010)
B.A. in English, Messiah College (2006-2010)
Timothy Mackie
Postdoctoral Fellow
Affiliations
Ph.D. in Molecular, Cell, and Developmental Biology, University of Pittsburgh (2012-2018)
B.S. in Biology, Messiah College (2006-2010)
B.A. in English, Messiah College (2006-2010)
Faculty
- Building:
- HSB
- Room:
- E410
- Box:
- 357280
- Phone:
- 205-616-1976
- Web Link:
- https://timothymackie.github.io/
Lab
- Building:
- HSB
- Room:
- E410
- Box:
- 357280
- Phone:
- 205-616-1976
- Web Link:
- https://depts.washington.edu/phcol/faculty/gardner.php
Timothy Mackie
Postdoctoral Fellow
Affiliations
Ph.D. in Molecular, Cell, and Developmental Biology, University of Pittsburgh (2012-2018)
B.S. in Biology, Messiah College (2006-2010)
B.A. in English, Messiah College (2006-2010)
Timothy Mackie
Postdoctoral Fellow
Affiliations
Ph.D. in Molecular, Cell, and Developmental Biology, University of Pittsburgh (2012-2018)
B.S. in Biology, Messiah College (2006-2010)
B.A. in English, Messiah College (2006-2010)
Timothy Mackie
Postdoctoral Fellow
Affiliations
Ph.D. in Molecular, Cell, and Developmental Biology, University of Pittsburgh (2012-2018)
B.S. in Biology, Messiah College (2006-2010)
B.A. in English, Messiah College (2006-2010)
Select Publications
Nadel, C.M., Mackie, T.D. and Gardner, R.G. (2019) Osmolyte accumulation regulates the SUMOylation and inclusion dynamics of the Cyc8-Tup1 transcription corepressor. PLoS Genetics, 15(4):e1008115.
Mackie, T.D., Kim B.Y., O’Donnell, A.F., Subramanya, A.R., Bain, D., Welling, P.A., and Brodsky, J.L. (2018) The endosomal trafficking factors CORVET and ESCRT negatively regulate plasma membrane residence of the Renal Outer Medullary Potassium channel (ROMK). Journal of Biological Chemistry, 293(9) 3201-3217.
Mackie, T.D. and Brodsky, J.L. (2018) Investigating Potassium Channels in Budding Yeast: A Genetic Sandbox. Genetics, 209(3) 637-650.
Publications
Timothy Mackie
Postdoctoral Fellow
Affiliations
Ph.D. in Molecular, Cell, and Developmental Biology, University of Pittsburgh (2012-2018)
B.S. in Biology, Messiah College (2006-2010)
B.A. in English, Messiah College (2006-2010)