Fields of Research
- Drug Discovery and Precision Medicine
- Intracellular Signaling
- Molecular Basis of Disease
I use mass spectrometry-based proteomics and chemical biology tools to identify cell signaling pathways in cancer that control metastasis and drug resistance. Ultimately, I aim to develop novel targeted drugs and combination treatments that can prevent tumor metastasis and reverse therapy resistance; such novel treatments will have a significant impact on therapy response and survival in cancer patient.
I use mass spectrometry, chemical biology and molecular biology tools to study cell signaling pathways dysregulated in human disease. Specifically, I seek to identify kinase-dependent cell signaling pathways that regulate proliferation, metastasis and drug resistance in cancer. Kinases that regulate such pathways pose novel targets for pharmacological intervention that could significantly improve therapy response and survival in cancer patients. To achieve my research goals, I am developing tools for selective enrichment and LC-MS analysis of protein kinases (kinobead-MS). These tools facilitate unbiased, system-wide measurements of protein kinase activity and, therefore, identify kinases that drive adverse cancer phenotypes. Using kinobead-MS I discovered that specific kinases control epithelial-mesenchymal transition (EMT) and drug resistance in hepatocellular carcinoma (HCC). Inhibition of these kinases reverses EMT and drug resistance, hence these kinases are promising target candidates for the future development of drug combination therapies.
Awards and Honors
Post-doctoral Fellowship, Deutsche Forschungsgemeinshaft (DFG)
Kinobead and Single-Shot LC-MS Profiling Identifies
Selective PKD Inhibitors. Golkowski M, Vidadala RS,
Lombard CK, Suh HW, Maly DJ, Ong SE. J Proteome Res.
2017 Mar 3;16(3):1216-1227. doi:
10.1021/acs.jproteome.6b00817. Epub 2017 Feb 3. PMID:
Studying mechanisms of cAMP and cyclic nucleotide
phosphodiesterase signaling in Leydig cell function with
phosphoproteomics. Golkowski M, Shimizu-Albergine M,
Suh HW, Beavo JA, Ong SE. Cell Signal. 2016 Jul;28
(7):764-78. doi: 10.1016/j.cellsig.2015.11.014. Epub 2015
Nov 28. PMID: 26643407.
Strategy for catch and release of azide-tagged biomolecules
utilizing a photolabile strained alkyne construct. Golkowski
M, Pergola C, Werz O, Ziegler T. Org Biomol Chem. 2012
Jun 21;10(23):4496-9. doi: 10.1039/c2ob25440a. Epub 2012May 9. PMID: 22573264