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Contact Information
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Ashley Tsue Profile

Fields of Research

  • Subnuclear Bodies
  • RNA-Protein Interactions

Research Summary

Study of RNA-protein interactions that drive subnuclear body assembly and function in health and disease


Research Statement

RNA-protein interactions underscore a diverse array of regulatory mechanisms throughout biology. When dysregulated, they contribute to an assortment of human pathologies, including neurodegenerative disorders, retroviral infections, and cancer. However, technical limitations have hindered the identification of RNA-protein interactions, limiting our understanding of fundamental cell biology, and leaving unexplored a potentially broad array of novel pharmacological targets. To overcome this problem, I am developing novel technologies that probe RNA-protein interactions within the cell. Our first test case is a noncoding RNA that scaffolds the nucleolus, an essential subnuclear body. The nucleolus is the site of ribosome biogenesis, a global metabolic regulator, and a hub for higher-order chromatin structure. It’s also causally implicated in a broad range of human pathologies, making it an attractive potential therapeutic target. Identifying key architectural RNA-protein interactions that drive nucleolar biogenesis, and which are dysregulated in disease states, can provide a potentially rich source of therapeutic targets.


Awards and Honors

Ashley Tsue

Graduate Student

Affiliations

UW Pharmacology Department

Funding Sources:
Pharmacological Sciences Training Program

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Why Pharmacology?

Ashley Tsue

Graduate Student

Affiliations

UW Pharmacology Department

Funding Sources:
Pharmacological Sciences Training Program

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Contact Information

Faculty

Building:
Health Sciences Building
Room:
K554
Box:
357750
Web Link:
http://depts.washington.edu/sheqlab/

Lab

Building:
Health Sciences Building
Room:
K554
Box:
357750
Phone:
206-543-0144
Web Link:
https://depts.washington.edu/phcol/faculty/shechner.php

Ashley Tsue

Graduate Student

Affiliations

UW Pharmacology Department

Funding Sources:
Pharmacological Sciences Training Program

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Courses

Ashley Tsue

Graduate Student

Affiliations

UW Pharmacology Department

Funding Sources:
Pharmacological Sciences Training Program

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Students/Postdocs

Ashley Tsue

Graduate Student

Affiliations

UW Pharmacology Department

Funding Sources:
Pharmacological Sciences Training Program

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Publications

Select Publications

Rusnac, D.-V., Lin, H.-C., Canzani, D., Tien, K.X., Hinds, T.R., Tsue, A.F., Bush, M.F., Yen, H.-C.S., and Zheng, N. (2018). Recognition of the Diglycine C-End Degron by CRL2KLHDC2 Ubiquitin Ligase. Mol. Cell 72, 813-822.e4.

Young, E.D., Strom, K., Tsue, A.F., Usset, J.L., MacPherson, S., McGuire, J.T., and Welch, D.R. (2018). Automated quantitative image analysis for ex vivo metastasis assays reveals differing lung composition requirements for metastasis suppression by KISS1. Clin. Exp. Metastasis 35, 77–86.

Publications

Ashley Tsue

Graduate Student

Affiliations

UW Pharmacology Department

Funding Sources:
Pharmacological Sciences Training Program

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